KMID : 0985420210430040214
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Laboratory Medicine and Quality Assurance 2021 Volume.43 No. 4 p.214 ~ p.216
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Comparison Study of Molecular Diagnostic Reagents for COVID-19 Pooling Test
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Seo Dong-Hee
Son Geun-Ju Kang Jeong-Woo
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Abstract
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In Korea, real-time reverse transcription-polymerase chain reaction (PCR) is performed on individual or pooled samples to diagnosis coronavirus disease 2019 (COVID-19). New developed LabGun COVID-19 ExoFast RT-PCR Kit (LabGenomics, Korea) was compared to PowerCheck SARS-CoV-2 Real-time PCR Kit (KogeneBiotech, Korea) for its properness about COVID-19 pooling test. LabGun kit showed 98.75% concordance for positive samples and 100% concordance for negative samples tested by PowerCheck kit. Agarose gel electrophoresis study revealed similar PCR products band intensities for two testing kits. In conclusion, LabGun kit could be used for COVID-19 pooling test.
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KEYWORD
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COVID-19, RT-PCR testing, Pooling test
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